(WHS-P87) ASSOCIATION OF TRYPTOPHAN/KYNURENINE METABOLITES WITH HEALING IN CHRONIC VENOUS LEG ULCERS
Friday, May 17, 2024
7:30 AM – 5:00 PM East Coast USA Time
Background: Chronic wound healing is a complex process that is still not well understood. The tryptophan (TRP)-L-Kynurenine (KYN) pathway increased scrutiny in wound healing. Indoleamine 2,3-dioxygenase (IDO) which mediates Tryptophan catabolism is highly active during pathological conditions including chronic wounds. We utilized metabolomics to investigate the biomarker involved in the TRP-L- KYN pathway in chronic venous leg ulcers (CVLUs) healing. The study aims to apply metabolomics to elucidate the TRP-L- KYN pathway associated with CVLUs wound healing.
Methods: The study was a longitudinal design, involving 60 serum samples collected from 30 older adult patients with CVLUs, who received weekly sharp debridement at a university wound clinic. The serum samples were collected at baseline, week 4, and week 8 (or at the time of wound closure). Liquid chromatography-mass spectrometry (LC-MS) metabolomics was used to analyze targeted metabolites. A Bayesian approach was employed to examine robust correlations between changes in metabolite values and linear healing slope.
Results: The mean age was 71.13 (±9.46); The healing group (n=23) demonstrated higher levels of mean TRP at baseline and overtime compared to the non-healing group. There was moderate support for a negative association between kynurenic acid and steeper healing slopes (r = -0.36, CrI = -0.62, -0.06).
Conclusion: Gaining a better understanding of the associations between the TRP-L- KYN pathway and the healing of CVLUs could help to clarify the links of inflammation with the rate and success of wound healing. Biomarker development focused on the TRP-L- KYN pathway could be pursued, if the associations are further supported by focused research studies.