Laboratory Research
Re-epithelialization is the process of restoring the barrier between the wound and the environment with a new epithelial layer. The cellular mechanisms by which this occurs are coordinated by a variety of regulatory proteins to promote the migration and proliferation of keratinocytes[1]. In this in vitro study, we evaluate the potential of a tri-layer lyophilized human amnion chorion membrane (LHACM*), which includes the amnion, intermediate, and chorion layers, to promote re-epithelialization by activating keratinocytes.
Methods:
LHACM was prepared using the PURION process, consisting of gentle cleansing, followed by lyophilization and terminal sterilization. Multiplex luminex assays were used to detect and quantify factors responsible for promoting keratinocyte migration and proliferation in LHACM extract. Effects on cellular proliferation and migration were assessed in HaCaT cells, an immortalized human keratinocyte cell line. Additionally, the expression levels of key signaling molecules associated with promotion of re-epithelialization were examined in order to determine potential mechanisms of action.
Results:
LHACM extract contains soluble factors associated with keratinocyte migration and proliferation. Treatment with LHACM extract supported significant enhancement in cellular proliferation of HaCaT cells in vitro. Additionally, the LHACM treated cells exhibited effects on cellular migration as indicated by accelerated closure of scratch wounds. Protein analysis of LHACM treated cells revealed increased expression of cJUN, a known promoter of keratinocyte migration[2].
Discussion:
The findings from this study suggest that LHACM activates keratinocytes, promoting both proliferation and migration in vitro. This research contributes valuable insights into the potential uses of LHACM to create an optimal wound healing environment, emphasizing its role in fostering keratinocyte function during the critical process of re-epithelialization.