Laboratory Research
H&E results confirmed the preservation of the native membrane’s structure at different layers. Lyophilization was preferable to the traditional dehydration methods for regulatory protein preservation. FT-AM maintained tested regulatory proteins, at similar levels to the native tissues. HDFa proliferation was increased when cells were exposed to the media conditioned with FT-AM. THP-1 macrophage inflammatory response in the presence of lipopolysaccharide (LPS) in media was attenuated when cells were exposed to LPS in the FT-AM conditioned media. Chemo-attractants in FT-AM promoted cell invasion and migration to FT-AM conditioned media from serum-free media.
Discussion:
FT-AM maintained physical structures, regulatory proteins, and biological properties that are known to aid external or internal tissue defects (e.g., wounds) by providing an extracellular matrix and promoting cell proliferation and migration while reducing inflammation. These outcomes indicate FT-AM can be used to enhance the healing cascade in external or internal tissue defects.